Putting the Pieces Together: Exons and piRNAs: A Dissertation
نویسنده
چکیده
BIOCHEMISTRY AND MOLECULAR PHARMACOLOGY Doctor of Philosophy Putting the Pieces Together: Exons and piRNAs by Christian K. Roy Analysis of gene expression has undergone a technological revolution. What was impossible 6 years ago is now routine. High-throughput DNA sequencing machines capable of generating hundreds of millions of reads allow, indeed force, a major revision toward the study of the genome’s functional output—the transcriptome. This thesis examines the history of DNA sequencing, measurement of gene expression by sequencing, isoform complexity driven by alternative splicing and mammalian piRNA precursor biogenesis. Examination of these topics is framed around development of a novel RNA-templated DNA-DNA ligation assay (SeqZip) that allows for efficient analysis of abundant, complex, and functional long RNAs. The discussion focuses on the future of transcriptome analysis, development and applications of SeqZip, and challenges presented to biomedical researchers by extremely large and rich datasets.
منابع مشابه
Telomerase discovery: The exciTemenT of puTTing TogeTher pieces of The puzzle
The story of telomerase discovery is a story of the thrill of putting pieces of a puzzle together to find something new. This story represents a paradigm for curiosity-driven research and, like many other stories of fundamental discovery, shows that important clinical insights can come from unlikely places. in this paper i describe the process of scientific discovery – at times frustrating, at ...
متن کاملHumpty Dumpty: Putting iBGP Back Together Again
Humpty Dumpty is the anthropomorphic nursery-rhyme egg broken into many pieces. Similarly, we have many pieces of measurement data to represent the current iBGP state. However, unlike the nurseryrhyme where the King’s men couldn’t put Humpty together again, we present a systematic approach to putting all the pieces of measured iBGP data together to obtain a more complete picture of a network’s ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2015